|
KMID : 0545120060160121928
|
|
Journal of Microbiology and Biotechnology
2006 Volume.16 No. 12 p.1928 ~ p.1934
|
|
|
Mediation of Rubradirin Resistance by ABC Transporters (RubT1) from Streptomyces achromogenes var. rubradiris NRRL3061
|
|
Lamichhane Janardan
Oh Tae-Jin
Lee Hei-Chan
Liou Kwang-Kyoung
Kim Chun-Gyu
Sohng Jae-Kyung
|
|
|
Abstract
|
|
|
|
The rubradirin biosynthetic gene cluster harbors 58 ORFs within a 105.6-kb sequence, which includes all of the genes responsible for the synthesis of rubradirin, as well as the primary genes relevant to regulatory, resistance, and transport functions. This gene cluster also harbors a resistance-mediating ABC transporter, RubT1, which is located at the most upstream position in the cluster. In the present study, RubT1 was expressed heterologously in E. coli, and the resistance affinity of RubT1 was determined by an antibacterial activity test, as well as by HPLC and ESI-MS analyses. Evidence clearly demonstrates that RubTl mediates rubradirin resistance as an ABC transporter.
|
|
|
KEYWORD
|
|
|
ABC transporter, membrane-spanning domain, resistance gene, rubradirin, S. achromogenes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|